麻豆传媒

NASA-funded research looks to isotope analysis rather than X-ray for measurement

News — TEMPE, Ariz. 鈥� Are your bones getting stronger or weaker? Right now, it鈥檚 hard to know. Scientists at Arizona State University and NASA are taking on this medical challenge by developing and applying a technique that originated in the Earth sciences. In a new study, this technique was more sensitive in detecting bone loss than the X-ray method used today, with less risk to patients. Eventually, it may find use in clinical settings, and could pave the way for additional innovative biosignatures to detect disease.

鈥淥steoporosis, a disease in which bones grow weaker, threatens more than half of Americans over age 50,鈥� explained Ariel Anbar, a professor in ASU鈥檚 Department of Chemistry and Biochemistry and the School of Earth and Space Exploration, and senior author of the study.

鈥淏one loss also occurs in a number of cancers in their advanced stages. By the time these changes can be detected by X-rays, as a loss of bone density, significant damage has already occurred,鈥� Anbar said. 鈥淎lso, X-rays aren鈥檛 risk-free. We think there might be a better way.鈥�

With the new technique, bone loss is detected by carefully analyzing the isotopes of the chemical element calcium that are naturally present in urine. Isotopes are atoms of an element that differ in their masses. Patients do not need to ingest any artificial tracers and are not exposed to any radiation, so there is virtually no risk, the authors noted.

The findings are presented in a paper published in the online Early Edition of the Proceedings of the National Academy of Sciences (PNAS) the week of May 28. It is titled 鈥淩apidly assessing changes in bone mineral balance using natural stable calcium isotopes.鈥�

鈥淭he paper suggests an exciting new approach to the problem,鈥� said Dr. Rafael Fonseca, chair of the Department of Medicine at the Mayo Clinic in Arizona, and a specialist in the bone-destroying disease multiple myeloma. Fonseca was not associated with the study but is partnering with the ASU team on collaborative research based on the findings.

鈥淩ight now, pain is usually the first indication that cancer is affecting bones. If we could detect it earlier by an analysis of urine or blood in high-risk patients, it could significantly improve their care,鈥� Fonseca said.

The new technique makes use of a fact well known to Earth scientists, but seldom used in biomedicine: Different isotopes of a chemical element can react at slightly different rates. When bones form, the lighter isotopes of calcium enter bone a little faster than the heavier isotopes. That difference, called 鈥渋sotope fractionation,鈥� is the key.

鈥淚nstead of isotopes of calcium, think about jelly beans,鈥� explained Jennifer Morgan, lead author of the study. 鈥淲e all have our favorite. Imagine a huge pile of jelly beans with equal amounts of six different kinds. You get to make your own personal pile, picking out the ones you want. Maybe you pick two black ones for every one of another color because you really like licorice. It鈥檚 easy to see that your pile will wind up with more black jelly beans than any other color. Therefore, the ratio of black to red or black to green will be higher in your pile than in the big one. That鈥檚 similar to what happens with calcium isotopes when bones form. Bone favors lighter calcium isotopes and picks them over the heavier ones.鈥�

Other factors, especially bone destruction, also come into play, making the human body more complicated than the jelly bean analogy. But 15 years ago, corresponding author Joseph Skulan, now an adjunct professor at ASU, combined all the factors into a mathematical model that predicted that calcium isotope ratios in blood and urine should be extremely sensitive to bone mineral balance.

鈥淏one is continuously being formed and destroyed,鈥� Skulan explained. 鈥淚n healthy, active humans, these processes are in balance. But if a disease throws the balance off then you ought to see a shift in the calcium isotope ratios.鈥�

The predicted effect on calcium isotopes is very small, but can be measured using sensitive mass spectrometry methods developed by Morgan as part of her doctoral work with Anbar, Skulan and co-author Gwyneth Gordon, an associate research scientist in the W.M. Keck Foundation Laboratory for Environmental Biogeochemistry at ASU. Co-author Stephen Romaniello, currently a doctoral student with Anbar at ASU, contributed an updated mathematical model.

The new study, funded by NASA, examined calcium isotopes in the urine of a dozen healthy subjects confined to bed (鈥渂ed rest鈥�) for 30 days at the University of Texas Medical Branch at Galveston鈥檚 Institute for Translational Sciences鈥揅linical Research Center. Whenever a person lies down, the weight-bearing bones of the body, such as those in the spine and leg, are relieved of their burden, a condition known as 鈥渟keletal unloading鈥�. With skeletal unloading, bones start to deteriorate due to increased destruction. Extended periods of bed rest induce bone loss similar to that experienced by osteoporosis patients, and astronauts.

鈥淣ASA conducts these studies because astronauts in microgravity experience skeletal unloading and suffer bone loss,鈥� said co-author Scott M. Smith, NASA nutritionist. 鈥淚t鈥檚 one of the major problems in human spaceflight, and we need to find better ways to monitor and counteract it. But the methods used to detect the effects of skeletal unloading in astronauts are also relevant to general medicine.鈥�

Lab analysis of the subjects鈥� urine samples at ASU revealed that the new technique can detect bone loss after as little as one week of bed rest, long before changes in bone density are detectable by the conventional approach, dual-energy X-ray absorptiometry (DEXA).

Importantly, it is the only method, other than DEXA, that directly measures net bone loss.

鈥淲hat we really want to know is whether the amount of bone in the body is increasing or decreasing鈥�, said Morgan.

Calcium isotope measurements seem poised to assume an important role in detecting bone disease 鈥� in space, and on Earth. The team is working now to evaluate the technique in samples from cancer patients.

鈥淭his is a 鈥榩roof-of-concept鈥� paper,鈥� explained Anbar 鈥淲e showed that the concept works as expected in healthy people in a well-defined experiment. The next step is to see if it works as expected in patients with bone-altering diseases. That would open the door to clinical applications.鈥�

However, the concept extends even beyond bone and calcium, the authors noted. Many diseases may cause subtle changes in element isotope abundances, or in the concentrations of elements. These sorts of signatures have not been systematically explored in the development of biosignatures of cancers and other diseases.

鈥淭he concept of inorganic signatures represents a new and exciting approach to diagnosing, treating and monitoring complex diseases such as cancer,鈥� stated Anna Barker, director of Transformative Healthcare Networks and co-director of the Complex Adaptive Systems Initiative in the Office of Knowledge Enterprise Development at ASU. Barker, who came to ASU after being deputy director of the National Cancer Institute, emphasized the simplicity of the approach compared to the challenges of deciphering complex genome-derived data, adding 鈥渢here is an opportunity to create an entirely new generation of diagnostics for cancer and other diseases.鈥�

The National Aeronautics and Space Administration Human Research Program and specifically the Human Health and Countermeasures Element and the Flight Analogs Project supported this work. Bed rest studies were supported in part by the National Center for Research Resources, National Institutes of Health.

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ARIZONA STATE UNIVERSITY ()Department of Chemistry/Biochemistry ()School of Earth and Space Exploration ()Research Matters ()Tempe, Arizona USA