Raman spectroscopy is an invaluable tool used by scientists for bioimaging and tissue diagnosis. In a study recently published in Lasers in Surgery and Medicine Journal, scientists and physicians in the Charles E. Schmidt College of Science at Florida Atlantic University have broadened the applicability of Raman spectroscopy by showing that it can be used to distinguish normal from cancerous residual skin tissue following high-powered laser ablation. This is the first time that Raman spectroscopy has been successfully used to detect cancerous tissue following laser ablation, setting the stage to use this technique as a guide for laser surgery. Scientists hope to one day employ Raman spectroscopy clinically in tandem with laser-ablative removal of skin cancers and possibly even other forms of cancers as well. 鈥淚 think the most important finding of this study for dermatologists and their patients is that it demonstrates the feasibility of a potentially much faster and more accurate mode of skin cancer treatment based entirely on laser technology,鈥� said Andrew C. Terentis, Ph.D., lead scientist of the study and an associate professor of chemistry and biochemistry in FAU鈥檚 Charles E. Schmidt College of Science.
Cancerous tissue is diagnosed using a Raman laser and the cancerous tissue is removed in a very precise, localized fashion using an ablation laser. This type of Raman-based technique for skin cancer diagnosis and treatment, although not yet fully developed, may one day obviate the need for a more costly and time-consuming treatment method like Mohs micrographic surgery, at least in some, if not all, clinical cases.
This study combined laser ablation for highly precise, hemostatic removal of cancerous tissue, with Raman spectroscopy to objectively and non-destructively probe the ablated tissue area in situ for any remaining cancer to remove. Raman spectra were collected from partially-ablated normal and squamous cell carcinoma samples and a spectral classification model based on principal component analysis with logistic regression correctly identified spectra from residual cancerous tissue with 95 percent sensitivity and 100 percent specificity.
鈥淪uccessful clinical implementation of the proposed surgical method could greatly enhance the speed and effectiveness of skin cancer treatment, especially if real-time analysis of the process were developed,鈥� said Terentis.
Mohs micrographic surgery is currently the gold-standard technique for skin cancer removal because it provides high cure rates while removing the least amount of healthy tissue. However, it is time consuming, resource-intensive, and the intraoperative evaluation of the excised tissue sections by histopathology is subjective. With Mohs surgery, the physician serves as surgeon, pathologist and reconstructive surgeon and relies on the accuracy of a microscope to trace and ensure removal of skin cancer down to its roots. This procedure allows dermatologists, trained in Mohs Surgery, to see beyond the visible disease, and to precisely identify and remove the entire tumor, leaving healthy tissue unharmed.
Terentis鈥� collaborators include co-author John Strasswimmer, M.D., Ph.D., affiliate faculty in FAU鈥檚 Charles E. Schmidt College of Medicine, and a skin cancer specialist and director of the Melanoma and Cutaneous Oncology Program at the Lynn Cancer Institute and Moffitt Cancer Network. Partial funding was provided by the Sinai Hospital of Detroit.
鈥淲hen a surgeon removes a cancer, whether it be with Mohs surgery for skin cancer or a surgeon using a robot in a modern operating room for abdominal cancer, the surgeon must rely on vision and touch to help decide initially how much tissue to remove,鈥� said Strawswimmer. 鈥淭his new work with professor Terentis sets the stage for us to have an automatic laser to vaporize cancer and the Raman spectroscopy to tell us when to stop the vaporization process. This is particularly important in areas that we can access with the laser beam such as the lungs or inside the liver that are otherwise very difficult to access with traditional surgery. We designed this study with skin cancer, because it is a very straightforward model study and the number of skin cancer patients is increasing at an exponential rate.鈥�
According to the American Academy of Dermatology, current estimates are that one in five Americans will develop skin cancer in their lifetime. More than 3.5 million cases of non-melanoma skin cancer, including basal cell carcinoma and squamous cell carcinoma, are diagnosed in more than 2 million people in the United States every year. It is estimated that 137,310 new cases of melanoma, 63,440 noninvasive (in situ) and 73,870 invasive, will be diagnosed in 2015. This year, it is estimated that one in 50 Americans will develop melanoma in their lifetime. - FAU -
About Florida Atlantic University:Florida Atlantic University, established in 1961, officially opened its doors in 1964 as the fifth public university in Florida. Today, the University, with an annual economic impact of $6.3 billion, serves more than 30,000 undergraduate and graduate students at sites throughout its six-county service region in southeast Florida. FAU鈥檚 world-class teaching and research faculty serves students through 10 colleges: the Dorothy F. Schmidt College of Arts and Letters, the College of Business, the College for Design and Social Inquiry, the College of Education, the College of Engineering and Computer Science, the Graduate College, the Harriet L. Wilkes Honors College, the Charles E. Schmidt College of Medicine, the Christine E. Lynn College of Nursing and the Charles E. Schmidt College of Science. FAU is ranked as a High Research Activity institution by the Carnegie Foundation for the Advancement of Teaching. The University is placing special focus on the rapid development of three signature themes 鈥� marine and coastal issues, biotechnology and contemporary societal challenges 鈥� which provide opportunities for faculty and students to build upon FAU鈥檚 existing strengths in research and scholarship. For more information, visit .
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