CRISPR creates 'invisible' male plants to boost crop yields
Chinese Academy of SciencesIn a new development, researchers have successfully created an efficient two-line system for hybrid seed production in Brassica napus (rapeseed) using CRISPR/Cas9 gene editing combined with the RUBY visual reporter. By targeting the BnaMS1 and BnaMS2 genes, they directly generated male-sterile lines from elite breeding materials, overcoming the traditional hurdles of propagation and sorting. The RUBY reporter enabled effortless identification of sterile and fertile seedlings, streamlining the entire process while significantly reducing labor and cost. This innovation promises to transform hybrid seed production, providing a scalable solution not only for rapeseed but also for other crops.