麻豆传媒

鈥淎ll of this dynamic variation between individuals and within individuals is really very surprising,鈥� says Whitehead Founding Member Rudolf Jaenisch, who is also a professor of biology at MIT. 鈥淲e don鈥檛 yet understand the significance of this. Is it functionally important and does it reflect, particularly in the brain, the history of neurons鈥攁ctivity states, for example? These are all interesting possibilities.鈥�

Methylation鈥攖he attachment of molecules, known as methyl groups, to DNA鈥攊s an epigenetic phenomenon that affects gene expression. Generally, methylated genes are turned off while unmethylated genes are active and ready for transcription. Although advances in sequencing technology have yielded informative methylation maps in a variety of tissues, such approaches only capture static 鈥渟napshots鈥� of methylation and are unable to reveal the dynamics of methylation in real time in tissues and individual cells.

Because of this limitation, researchers have theorized that inherited methylation, also referred to as parental imprinting, largely remains stable throughout development, except during two important developmental milestones: after fertilization and during the creation of sperm and egg cells. Altered gene imprinting at other times has been associated with developmental disorders and cancer.

To investigate active methylation in individual cells, Jaenisch lab postdoctoral researcher Yonatan Stelzer developed a reporter system that tracks genomic methylation in real-time. When a target gene is unmethylated, the reporter is also unmethylated, triggering expression of a glowing protein that illuminates the cell. When the target is methylated, so too is the reporter. The glowing protein is then unexpressed, leaving the cell dark. As the target gene鈥檚 methylation changes, so does the reporter鈥檚.

In the latest research, described online this week in the journal Cell Reports, Stelzer used the reporter system in mice to discover that imprinted methylation in developing and adult tissues is actively regulated rather than merely maintained in stable fashion.

鈥淲hat we see with the reporter in adult tissues is very surprising and much more complex than we had thought,鈥� says Stelzer, who is a co-author of the Cell Reports paper. 鈥淭he regulation of imprinted methylation results in these very consistent patterns in different tissues and even in a cell-type dependent manner. In the case of neural cells, it means that imprinted methylation is dynamically shaping the adult brain over time and could play a role in aging. Because this imprinting affects hundreds of genes that are non-coding, including microRNAs and non-coding RNAs, it鈥檚 a very interesting fine-tuning mechanism for the dosage of gene expression in the brain and elsewhere in the body.鈥�

Although Stelzer notes that the results of his latest research are indeed exciting, they are just a beginning.

鈥淥ur work is the first observation of these changes,鈥� he says. 鈥淣ow we need to understand their functional consequences and the mechanism that regulates these changes in methylation.鈥�

This work was supported by the National Institutes of Health (NIH grant HD 045022), the Human Frontier Science Program, and the Brain and Behavior Research Foundation (grant 22950). Jaenisch is an advisor to Stemgent and a cofounder of Fate Therapeutics and Fulcrum Therapeutics.

Written by Nicole Giese Rura

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Rudolf Jaenisch's primary affiliation is with Whitehead Institute for Biomedical Research, where his laboratory is located and all his research is conducted. He is also a professor of biology at Massachusetts Institute of Technology.

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Full Citation:

鈥淧arent-of-origin DNA methylation dynamics during mouse development鈥�

Cell Reports, online September 20, 2016.

Yonatan Stelzer (1), Hao Wu (1), Yuelin Song (1,2), Chikdu Shivalila (1,2), Styliani Markoulaki (1), and Rudolf Jaenisch (1,2).

1. Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA2. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA